This page will allow you to propose a research project to be performed at the CryoEM Center.
The CryoEM Center is equipped with four state of the art intermediate voltage electron cryomicroscopes, sample vitrification robots and a wide range of computational resources all devoted towards our mission of advancing the technology of electron cryomicroscopy and applying this technology to problems of biological significance. We have one phase-plate microscope, and 3 of 4 instruments are equipped with a direct-detection CMOS camera. Our projects span a wide range of topics from macromolecular structural biology, nanobiology, virology, microbiology and cell biology. We have a variety of techniques for examining specimens ranging in size from 10 nm to a micron with resolutions in some cases at subnanometer details. Available techniques include single particle reconstruction, tomography, helical reconstruction and 2-D crystallography. We are involved in work ranging from heavily collaborative projects to strictly service projects. For a collaborative project, our center staff devote significant amounts of effort in carrying out or assisting the projects at one or more phases in the project pipeline from specimen preparation to structural interpretation. For service projects, the users make use of our experimental facility or software with minimum assistance beyond initial training from our staff. Center staff are generally co-authors in the publications in collaborative projects whereas co-authorship is not obligatory for service projects. In either case, NIH support (grant number P41GM103832) must be acknowledged in any publication using the NCMI facility.
If you have confidentiality concerns, or would like to discuss the general feasibility of your project prior to submitting a proposal, you are welcome to email Steve Ludtke (firstname.lastname@example.org).
If you have a proposal you think would be appropriate for the NCMI, please submit detailed information on the form below. Once submitted, your proposal will be evaluated by the CryoEM Center director, and in some cases outside consultants. We will contact you within two weeks with information on how we can proceed. If the project appears promising we typically begin with an initial set of experiments to assess feasibility.
Before filling out the form, please note some basic requirements:
For single particle reconstruction:
The proposal requires the following information: