Mr T is a tomography software written in Python.Mr T uses the JAMES libraries to control the electron microscope and detector. Image processing is accomplished with EMAN.

Software dependencies:
  • 5-axis control on FasTEM (JEOL)
  • Digital Micrograph (Gatan)
  • EMAN
  • Python
  • Python Numeric
  • Python Imaging Library

  • References

    Chang JT, Marsh M, Rixon F, Chiu W. (2005)
    Mr T: Automated Electron Cryotomography for JEOL 2010F TEM.
    Microscopy and Microanalysis 11 (Suppl 2): 308-309 (link)

    Marsh M, Chang J, Chen J, Lopez J, Chiu W. (2005)
    Three-Dimensional Architecture of Platelets in its Native State by Electron Cryotomography
    Blood 106(11) (link)

    Cruciano A, Schmid MF, Marsh MP, Chang J, Thompson LR, Sullivan MB, Chisholm SW, Chiu W. (2006)
    3-Dimensional Reconstruction of Cyanophage P-SS2 using Cryo-Electron Tomography
    4th International Congress on Electron Tomography, San Diego, CA.

    Chang JT, Schmid MF, Rixon FJ, Chiu W. (2007)
    Electron Cryo-Tomography Reveals the Portal in the Herpesvirus Capsid.
    Journal of Virology 81(4): 2065-2068


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