This workshop will cover the following topics: the principle of the MPSA (Multi-Path Simulated Annealing) algorithm, the principle of cross common line, virus reconstruction with icosahedral symmetry enforced using MPSA, and virus reconstruction without symmetry enforced using MPSA procedure. MPSA is a newly developed optimization algorithm. It is able to search virus particle image orientation in 5-dimensional space (3 for rotation, 2 for center) at the same time using very small amount of CPU time. The MPSA procedure is build based on the cross common line principle. This procedure works in Fourier space. It iteratively refines virus density map from scratch (without known initial model required) to high resolution structure.


1. Liu, X., Jiang, W., Jakana, J. & Chiu, W. Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm. J Struct Biol 160, 11-27 (2007).

2. Crowther, R. A., DeRosier, D. J. & Klug, A. The reconstruction of a three-dimensional structure from projections and its application to electron microscopy. Proc Roy Soc Lond A 317, 319-340 (1970).

3. Crowther, R.A., Amos, L.A., Finch, J.T., DeRosier, D.J., Klug, A. Three dimensional reconstructions of spherical viruses by Fourier synthesis from electron micrographs. Nature 226, 421äóñ425 (1970).

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